Research Technician I

Overview: Fred Hutchinson Cancer Center is an independent, nonprofit organization providing adult cancer treatment and groundbreaking research focused on cancer and infectious diseases. Based in Seattle, Fred Hutch is the only National Cancer Institute-designated cancer center in Washington. With a track record of global leadership in bone marrow transplantation, HIV/AIDS prevention, immunotherapy and COVID-19 vaccines, Fred Hutch has earned a reputation as one of the worlds leading cancer, infectious disease and biomedical research centers. Fred Hutch operates eight clinical care sites that provide medical oncology, infusion, radiation, proton therapy and related services, and network affiliations with hospitals in five states. Together, our fully integrated research and clinical care teams seek to discover new cures to the worlds deadliest diseases and make life beyond cancer a reality. At Fred Hutch we value collaboration, compassion, determination, excellence, innovation, integrity and respect. Our mission is directly tied to the humanity, dignity and inherent value of each employee, patient, community member and supporter. Our commitment to learning across our differences and similarities make us stronger. We seek employees who bring different and innovative ways of seeing the world and solving problems. The Srivastava Lab at Fred Hutch is looking for a highly motivated Research Technician I to support the development of CAR-T cell immunotherapy for cancer. The successful candidate will assist with design, execution, and analysis of pre-clinical studies using murine tumor models to study strategies for improving CAR-T cell therapy. She/he should be capable of handling mice, maintaining detailed laboratory records, analyzing data, and reporting experimental results in a fast-paced environment. Responsibilities will include working both independently and as a member of a team, providing technical assistance with ongoing research projects. Responsibilities: Primary Duties and Responsibilities

• Perform sterile tissue culture techniques, including primary T cell culture, growing and titering of retrovirus and lentivirus, and transduction of murine cells with retroviral/lentiviral vectors
• Assist with in vivo murine tumor model experiments, including: monitoring weight, body condition score, and tumor growth; administering injections (intravenous, intraperitoneal, and subcutaneous)
• Dissect and process various tissues, including spleen, peripheral lymph nodes, bone marrow, lungs, and tumors
• Analyze cell phenotype and function from in vitro and in vivo experiments using multi-parameter flow cytometry
• Prepare samples for analysis by next-generation sequencing methods, including single-cell RNAseq and CUT&Tag
• Molecular biology techniques, including bacterial plasmid preps, PCR, and cloning
• Maintain detailed records, schedule mouse studies and analyze data
• Provide basic support to the laboratory
• Perform general lab/equipment maintenance, keep stock of lab supplies, and assist the lab manager with general upkeep of the lab
• Computer skills: Microsoft Excel, PowerPoint, Prism (Graphpad), FlowJo

Qualifications: MINIMUM QUALIFICATIONS:

• Bachelor of Science degree in a biological science
• Prior experience working in a wet lab environment
• Detail-oriented, well-organized, and have good attention to detail
• Ability to communicate effectively and keep accurate records of work performed
• Ability to work on a team
• High level of self-motivation and initiative

PREFERRED QUALIFICATIONS:

• Prior experience with sterile tissue culture and mouse handling
• Prior exposure to immunology (this can be from coursework or prior lab experience.)

The hourly pay range for this position is from $22.87 to $32.56 and pay offered will be based on experience and qualifications. Fred Hutchinson Cancer Center offers employees a comprehensive benefits package designed to enhance health, well-being, and financial security. Benefits include medical/vision, dental, flexible spending accounts, life, disability, retirement, family life support, employee assistance program, onsite health clinic, tuition reimbursement, paid vacation (12-22 days per year), paid sick leave (12-25 days per year), paid holidays (13 days per year), paid parental leave (up to 4 weeks), and partially paid sabbatical leave (up to 6 months). Additional Information: We are proud to be an Equal Employment Opportunity (EEO) and Vietnam Era Veterans Readjustment Assistance Act (VEVRAA) Employer. We do not discriminate on the basis of race, color, religion, creed, ancestry, national origin, sex, age, disability (physical or mental), marital or veteran status, genetic information, sexual orientation, gender identity, political ideology, or membership in any other legally protected class. We encourage individuals with diverse backgrounds to apply and desire priority referrals of protected veterans. If due to a disability you need assistance/and or a reasonable accommodation during the application or recruiting process, please send a request to Human Resources at hrops@fredhutch.org or by calling 206-667-4700.